CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

Technique for Controlling Spread of Limnotic Oncomelania   总被引：1，自引：0，他引：1  

李大美  王祥三  LAI Yonggen 《中国海洋湖沼学报》2003,21(3):251-260

Schistosomiasis is a parasitic disease mostly found in areas along the Changjiang River of China. The disease is spread solely through an intermediary named oncomelania, so its spread of schistosomiasis can be controlled by properly designing water intakes which prevent oncomelania from entering farming land or residential areas. This paper reports a successful design process and a new oncomelania-free intake device. The design of the new intake is based on a sound research program in which extensive experimental studies were carried out to gain knowledge of oncomelania eco-hydraulic behavior and detailed flow field information through CFD simulation.  相似文献   

Report on Pontoniinae shrimps （Crustacea：Decapoda）collected by Joint Chinese—German Marine Biology Expeditions to Hainan lsland ,South China Sea：Ⅱ.Harpiliopsis,Ischnopontonia,Jocaste,Palae—mone     

李新正  刘瑞玉 《中国海洋湖沼学报》2003,21(2):154-165

The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island.  相似文献   

天文光干涉与综合孔径技术的发展     

郭红锋 《紫金山天文台台刊》2003,(1)

本文简单介绍了光干涉与综合孔径技术发展的历史、国际上用于天文观测的一些著名光干涉仪的技术特点和进展、以及近年来我国开展的与光干涉 ,综合孔径技术有关的工作 ,最后简要介绍了在光学综合孔径技术领域所发展的光纤与集成光学等新技术  相似文献   

APPLICATION OF GEOGRAPHICAL PARAMETER DATABASE TO ESTABLISHMENT OF UNIT POPULATION DATABASE   总被引：1，自引：1，他引：0  

DONG Chun  ZHAO Rong  LIU Ji-ping  WANG Gui-xin  WU Xi-zhi 《中国地理科学(英文版)》2003,13(1):34-38

Now GIS is turning into a good tool in handling geographical, economical, and population data, so we can obtain more and more information from these data. On the other hand, in some cases, for a calamity, such as hurricane, earthquake, flood, drought etc., or a decision-making, such as setting up a broadcasting transmitter, building a chemical plant etc., we have to evaluate the total population in the region influenced by a calamity or a project. In this paper, a method is put forward to evaluate the population in such special region. Through exploring the correlation of geographical parameters and the distribution of people in the same region by means of quantitative analysis and qualitative analysis, unit population database (1km× 1km) is established. In this way, estimating the number of people in a special region is capable by adding up the population in every grid involved in this region boundary. The geographical parameters are obtained from topographic database and DEM database on the scale of  相似文献   

丽江高美古台址和2m级望远镜项目(英文)     

陈东  王建成  许骏  罗国权  谭徽松  刘忠  张柏荣 《天文研究与技术》2003,(Z1)

自 1992年起云南天文台开始了在云南省西北部的天文选址。丽江高美古最终被选择作为未来中国地基天文光学观测的新址点。并且 ,在国家科技部 ,云南省政府 ,以及中科院的支持下 ,2m级天文望远镜项目在 2 0 0 0年 12月获得立项。在文中 ,我们简要的介绍了高美古台址的情况 ,并给出了 2 .4m望远镜的详细情况 ,以及其他相关情况。  相似文献   

EMERGY ANALYSIS OF GRAIN PRODUCTION SYSTEM IN JIANGSU AND SHAANXI PROVINCES     

LIUXin-wei CHENBai-ming ZHANGDing-xiang 《中国地理科学(英文版)》2004,14(3):209-214

Efficiency and sustainability of grain production are now important in China. In this study, the grain production systems of Jiangsu and Shaanxi Provinces were compared, to analyze their efficiency and sustainability in terms of utilization of natural resources, inputs of purchased energy and materials, and outputs. Flows of energy and materials between environment and human society were identified, and the natural and human work involved in generating inputs as materials or energy were valued in terms of equivalent amount of solar energy required for their production using emergy method. The results showed environmental resources were continually playing a less important role in the systems, when inorganic subsidiary emergy inputs increased drastically while organic ones decreased or increased little. Deterioration of input emergy structure affected the systems‘ efficiency and sustainability, resulting in emergy investment ratios and environmental loading ratios increasing while yield ratios and sustainability indices decreasing. In general, efficiency and sustainability of grain production in Jiangsu are worse than those in Shaanxi. This analysis also suggested that inorganic subsidiary emergy should be introduced properly, and peasants in Jiangsu should utilize natural conditions wisely while those in Shaanxi pay enough attention to soil and surface water conservation.  相似文献   

CORRESPONDENCE ANALYSIS ON IMAGES OF JIANGXI PROVINCE AS A TOURIST DESTINATION   总被引：4，自引：0，他引：4  

MAO Duan-qian ZHANG Jie BAO Hao-sheng 《中国地理科学(英文版)》2005,15(3):275-280

1IN T R O D U C T IO NAn image isa setofbelief,s ideasand impressionsthatapersonholdsaboutan object(KOTLER,1991).Similar-lydefinitionistheimage of a destinationas thesum ofbelief,sideasand impressionsthatpeoplehave ofa placeor destination(CROMPTON,1979;KO…  相似文献   

EFFECT OF SULPHATE-REDUCING BACTERIA ON ELECTRO-CHEMICAL CORROSION BEHAVIOR OF 16Mn STEEL IN SEA MUD     

张经磊  侯保荣  郭公玉  孙虎元  徐岩 《中国海洋湖沼学报》2001,19(1):87-90

ＩＮＴＲＯＤＵＣＴＩＯＮＩｔｗａｓｇｅｎｅｒａｌｌｙｃｏｎｓｉｄｅｒｅｄｐｒｅｖｉｏｕｓｌｙｔｈａｔｔｈｅｃｏｒｒｏｓｉｏｎｏｆｓｔｅｅｌｉｎｓｅａｍｕｄｗａｓｎｏｔｓｅｒｉｏｕｓ.Ｈｏｗｅｖｅｒ,ｉｔｗａｓｌａｔｅｒｆｏｕｎｄｔｈａｔｔｈｅｃｏｒｒｏｓｉｏｎｒａｔｅｏｆｓｔｅｅｌｉｎｓｏｍｅｓｅａｍｕｄｗａｓｈｉｇｈｅｒｔｈａｎｔｈａｔｉｎｓｅａｗａｔｅｒ(Ｋｉｎｇ,1980;Ｓｅｈｍｉｄｅ,1982;Ｇｕｏｅｔａｌ.,1997;Ｈｏｕ,1998).Ｔｈｅｒｅａｒｅｓｏｍｅｒｅｐｏｒｔｓｔｈａｔｔｈｅｃｏｒｒｏｓｉｏｎｒ…  相似文献   

Petroleum Hydrocarbon Distribution Features in Water and Sediment off Fujian Shore   总被引：1，自引：0，他引：1  

王宪  徐鲁荣  李文权  李凌云  钱爱红 《中国海洋湖沼学报》2003,21(2):187-192

This study on the distribution features of petroleum hydrocarbon in water and sedi-ment off the Fujian shore using data obtained from the baseline research on oceanic contamination in Fujian showed that:during the research preiod,petroleum hydrocarbon concentrations in water varied from 5.77μg/L to 37.28μg/L,averaged 14.48 μg/L;was lower in the wet seasson than in the dry season;and was highest in the Minjiang Estuary and Jiulong Estuary in both seasons ,The petorleum hydrocarbon concentrations in shore sediment varied from 14.48 mg/kg to 784.36 mg/kg,averaged 133.3 mg/kg ,and was closely related to sediment types (granularity).  相似文献